pe conjugated anti cd86  (Elabscience Biotechnology)

Journal: Frontiers in Immunology

Article Title: The transcription factor TSHZ3 promotes tumor immunosuppression and inhibits metastasis in lung adenocarcinoma

doi: 10.3389/fimmu.2025.1519815

Figure Lengend Snippet: Co-culturing THP-1 macrophages with A549-TSHZ3 cells enhances macrophage chemotaxis and A549 cell apoptosis. THP-1 macrophages were co-cultured with either A549-TSHZ3-NC or A549-TSHZ3 cells, and then the bottom surfaces of THP-1 macrophages were observed. (B , C) CCR2 and CD86 expression in THP-1 macrophages were assayed using flow cytometry after co-culture. (D) RT-PCR was performed to assess CCL2 gene expression in A549-TSHZ3-NC and A549-TSHZ3 cells. (E) Apoptotic cells in A549-TSHZ3-NC and A549-TSHZ3 groups were assessed using flow cytometry. (F) CCL2 and apoptosis-related protein expression were analyzed via Western blotting. * p < 0.05; ns, not significant. Data are shown as mean ± SEM, representative of three independent experiments with similar results.

Article Snippet: The macrophage cells from the co-culture system were harvested and stained with PE-conjugated anti-CD86 antibody (eBioscience, Waltham, USA) or Allophycocyanin (APC)-conjugated anti-CCR2 antibody (Biolegend, San Diego, USA) in PBS for 30 min in the dark.

Techniques: Chemotaxis Assay, Cell Culture, Expressing, Flow Cytometry, Co-Culture Assay, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Western Blot